1 00:00:02,159 --> 00:00:03,750 hello thank you for checking out my 2 00:00:03,750 --> 00:00:03,760 hello thank you for checking out my 3 00:00:03,760 --> 00:00:04,470 hello thank you for checking out my lightning talk 4 00:00:04,470 --> 00:00:04,480 lightning talk 5 00:00:04,480 --> 00:00:06,230 lightning talk i'm rachel tucker an undergraduate 6 00:00:06,230 --> 00:00:06,240 i'm rachel tucker an undergraduate 7 00:00:06,240 --> 00:00:07,990 i'm rachel tucker an undergraduate student at the university of florida 8 00:00:07,990 --> 00:00:08,000 student at the university of florida 9 00:00:08,000 --> 00:00:09,509 student at the university of florida and i've been studying martian regolith 10 00:00:09,509 --> 00:00:09,519 and i've been studying martian regolith 11 00:00:09,519 --> 00:00:11,110 and i've been studying martian regolith as a substrate for foreign planetary 12 00:00:11,110 --> 00:00:11,120 as a substrate for foreign planetary 13 00:00:11,120 --> 00:00:12,470 as a substrate for foreign planetary horticulture 14 00:00:12,470 --> 00:00:12,480 horticulture 15 00:00:12,480 --> 00:00:14,789 horticulture why do regular studies matter human 16 00:00:14,789 --> 00:00:14,799 why do regular studies matter human 17 00:00:14,799 --> 00:00:16,470 why do regular studies matter human space exploration is increasing in 18 00:00:16,470 --> 00:00:16,480 space exploration is increasing in 19 00:00:16,480 --> 00:00:17,590 space exploration is increasing in duration and distance 20 00:00:17,590 --> 00:00:17,600 duration and distance 21 00:00:17,600 --> 00:00:19,349 duration and distance which poses a greater need for in-situ 22 00:00:19,349 --> 00:00:19,359 which poses a greater need for in-situ 23 00:00:19,359 --> 00:00:20,950 which poses a greater need for in-situ resource utilization in order to 24 00:00:20,950 --> 00:00:20,960 resource utilization in order to 25 00:00:20,960 --> 00:00:22,950 resource utilization in order to maximize available resources 26 00:00:22,950 --> 00:00:22,960 maximize available resources 27 00:00:22,960 --> 00:00:25,109 maximize available resources a regolith simulant offers a comparable 28 00:00:25,109 --> 00:00:25,119 a regolith simulant offers a comparable 29 00:00:25,119 --> 00:00:26,470 a regolith simulant offers a comparable substrate in order to study the 30 00:00:26,470 --> 00:00:26,480 substrate in order to study the 31 00:00:26,480 --> 00:00:28,470 substrate in order to study the potential of martian regolith as a local 32 00:00:28,470 --> 00:00:28,480 potential of martian regolith as a local 33 00:00:28,480 --> 00:00:30,870 potential of martian regolith as a local resource for crop production on mars 34 00:00:30,870 --> 00:00:30,880 resource for crop production on mars 35 00:00:30,880 --> 00:00:32,229 resource for crop production on mars why do we want to look at mars global 36 00:00:32,229 --> 00:00:32,239 why do we want to look at mars global 37 00:00:32,239 --> 00:00:33,910 why do we want to look at mars global simulant 1. 38 00:00:33,910 --> 00:00:33,920 simulant 1. 39 00:00:33,920 --> 00:00:35,590 simulant 1. other martian simulants are available 40 00:00:35,590 --> 00:00:35,600 other martian simulants are available 41 00:00:35,600 --> 00:00:36,950 other martian simulants are available such as jsc mars 1 42 00:00:36,950 --> 00:00:36,960 such as jsc mars 1 43 00:00:36,960 --> 00:00:39,190 such as jsc mars 1 and mojave mars simulink both are 44 00:00:39,190 --> 00:00:39,200 and mojave mars simulink both are 45 00:00:39,200 --> 00:00:40,630 and mojave mars simulink both are sourced from natural environments and 46 00:00:40,630 --> 00:00:40,640 sourced from natural environments and 47 00:00:40,640 --> 00:00:42,869 sourced from natural environments and have been widely studied in literature 48 00:00:42,869 --> 00:00:42,879 have been widely studied in literature 49 00:00:42,879 --> 00:00:45,190 have been widely studied in literature mars global simulant 1 is a recipe 50 00:00:45,190 --> 00:00:45,200 mars global simulant 1 is a recipe 51 00:00:45,200 --> 00:00:46,869 mars global simulant 1 is a recipe created by excellence labs 52 00:00:46,869 --> 00:00:46,879 created by excellence labs 53 00:00:46,879 --> 00:00:49,510 created by excellence labs to mimic the mineral chemical volatile 54 00:00:49,510 --> 00:00:49,520 to mimic the mineral chemical volatile 55 00:00:49,520 --> 00:00:51,270 to mimic the mineral chemical volatile and spectral properties of the rockness 56 00:00:51,270 --> 00:00:51,280 and spectral properties of the rockness 57 00:00:51,280 --> 00:00:52,790 and spectral properties of the rockness regolith at gale crater which is the 58 00:00:52,790 --> 00:00:52,800 regolith at gale crater which is the 59 00:00:52,800 --> 00:00:56,150 regolith at gale crater which is the best characterized regolith to date 60 00:00:56,150 --> 00:00:56,160 best characterized regolith to date 61 00:00:56,160 --> 00:00:58,869 best characterized regolith to date in our experiment we wanted to determine 62 00:00:58,869 --> 00:00:58,879 in our experiment we wanted to determine 63 00:00:58,879 --> 00:00:59,189 in our experiment we wanted to determine what 64 00:00:59,189 --> 00:00:59,199 what 65 00:00:59,199 --> 00:01:02,709 what low concentrations of the mars global 66 00:01:02,709 --> 00:01:02,719 low concentrations of the mars global 67 00:01:02,719 --> 00:01:03,349 low concentrations of the mars global stimulant 68 00:01:03,349 --> 00:01:03,359 stimulant 69 00:01:03,359 --> 00:01:06,310 stimulant how that could affect crops and so we 70 00:01:06,310 --> 00:01:06,320 how that could affect crops and so we 71 00:01:06,320 --> 00:01:06,870 how that could affect crops and so we tested 72 00:01:06,870 --> 00:01:06,880 tested 73 00:01:06,880 --> 00:01:08,469 tested three different concentrations of 74 00:01:08,469 --> 00:01:08,479 three different concentrations of 75 00:01:08,479 --> 00:01:10,310 three different concentrations of regolith relative 76 00:01:10,310 --> 00:01:10,320 regolith relative 77 00:01:10,320 --> 00:01:13,830 regolith relative to an inert arcellite substrate base 78 00:01:13,830 --> 00:01:13,840 to an inert arcellite substrate base 79 00:01:13,840 --> 00:01:17,429 to an inert arcellite substrate base we tested 10 25 and and 50 percent 80 00:01:17,429 --> 00:01:17,439 we tested 10 25 and and 50 percent 81 00:01:17,439 --> 00:01:19,270 we tested 10 25 and and 50 percent mars global stimulant one relative to 82 00:01:19,270 --> 00:01:19,280 mars global stimulant one relative to 83 00:01:19,280 --> 00:01:20,550 mars global stimulant one relative to the arcellite 84 00:01:20,550 --> 00:01:20,560 the arcellite 85 00:01:20,560 --> 00:01:23,030 the arcellite during growth we we collected daily 86 00:01:23,030 --> 00:01:23,040 during growth we we collected daily 87 00:01:23,040 --> 00:01:24,789 during growth we we collected daily collected data daily for 88 00:01:24,789 --> 00:01:24,799 collected data daily for 89 00:01:24,799 --> 00:01:26,870 collected data daily for radical emergence cotyledon emergence 90 00:01:26,870 --> 00:01:26,880 radical emergence cotyledon emergence 91 00:01:26,880 --> 00:01:28,710 radical emergence cotyledon emergence first true leaves budding 92 00:01:28,710 --> 00:01:28,720 first true leaves budding 93 00:01:28,720 --> 00:01:30,870 first true leaves budding and at 10 days after planting we 94 00:01:30,870 --> 00:01:30,880 and at 10 days after planting we 95 00:01:30,880 --> 00:01:33,030 and at 10 days after planting we harvested and collected fresh weight 96 00:01:33,030 --> 00:01:33,040 harvested and collected fresh weight 97 00:01:33,040 --> 00:01:37,270 harvested and collected fresh weight dry weight height and widest diameter 98 00:01:37,270 --> 00:01:37,280 dry weight height and widest diameter 99 00:01:37,280 --> 00:01:39,590 dry weight height and widest diameter we planted 25 seeds for each of the 100 00:01:39,590 --> 00:01:39,600 we planted 25 seeds for each of the 101 00:01:39,600 --> 00:01:40,870 we planted 25 seeds for each of the following cultivars 102 00:01:40,870 --> 00:01:40,880 following cultivars 103 00:01:40,880 --> 00:01:43,590 following cultivars daikon radish amara mustard extra dwarf 104 00:01:43,590 --> 00:01:43,600 daikon radish amara mustard extra dwarf 105 00:01:43,600 --> 00:01:44,550 daikon radish amara mustard extra dwarf bok choy 106 00:01:44,550 --> 00:01:44,560 bok choy 107 00:01:44,560 --> 00:01:46,630 bok choy buckwheat red russian kale and 108 00:01:46,630 --> 00:01:46,640 buckwheat red russian kale and 109 00:01:46,640 --> 00:01:49,270 buckwheat red russian kale and outrageous red romaine 110 00:01:49,270 --> 00:01:49,280 outrageous red romaine 111 00:01:49,280 --> 00:01:52,389 outrageous red romaine pictured here is a top view of 3 112 00:01:52,389 --> 00:01:52,399 pictured here is a top view of 3 113 00:01:52,399 --> 00:01:55,590 pictured here is a top view of 3 5 7 and 10 days after planting of the 114 00:01:55,590 --> 00:01:55,600 5 7 and 10 days after planting of the 115 00:01:55,600 --> 00:01:57,510 5 7 and 10 days after planting of the control group in the top row 116 00:01:57,510 --> 00:01:57,520 control group in the top row 117 00:01:57,520 --> 00:02:01,429 control group in the top row 10 25 and the 50 concentration of mars 118 00:02:01,429 --> 00:02:01,439 10 25 and the 50 concentration of mars 119 00:02:01,439 --> 00:02:03,910 10 25 and the 50 concentration of mars global stimulant 120 00:02:03,910 --> 00:02:03,920 global stimulant 121 00:02:03,920 --> 00:02:06,069 global stimulant as you can see as the concentration of 122 00:02:06,069 --> 00:02:06,079 as you can see as the concentration of 123 00:02:06,079 --> 00:02:07,990 as you can see as the concentration of mars global stimulant increases the 124 00:02:07,990 --> 00:02:08,000 mars global stimulant increases the 125 00:02:08,000 --> 00:02:12,229 mars global stimulant increases the amount of vegetative tissue decreases 126 00:02:12,229 --> 00:02:12,239 127 00:02:12,239 --> 00:02:13,750 picture here is a graph for each of the 128 00:02:13,750 --> 00:02:13,760 picture here is a graph for each of the 129 00:02:13,760 --> 00:02:15,990 picture here is a graph for each of the cultivars and the x-axis is the number 130 00:02:15,990 --> 00:02:16,000 cultivars and the x-axis is the number 131 00:02:16,000 --> 00:02:17,190 cultivars and the x-axis is the number of days after planting 132 00:02:17,190 --> 00:02:17,200 of days after planting 133 00:02:17,200 --> 00:02:19,830 of days after planting and in the y-axis is the percent of 134 00:02:19,830 --> 00:02:19,840 and in the y-axis is the percent of 135 00:02:19,840 --> 00:02:21,110 and in the y-axis is the percent of seeds planted 136 00:02:21,110 --> 00:02:21,120 seeds planted 137 00:02:21,120 --> 00:02:22,470 seeds planted as you can see here for radical 138 00:02:22,470 --> 00:02:22,480 as you can see here for radical 139 00:02:22,480 --> 00:02:24,229 as you can see here for radical emergence there was a large difference 140 00:02:24,229 --> 00:02:24,239 emergence there was a large difference 141 00:02:24,239 --> 00:02:25,910 emergence there was a large difference in each of the six cultivars between the 142 00:02:25,910 --> 00:02:25,920 in each of the six cultivars between the 143 00:02:25,920 --> 00:02:27,030 in each of the six cultivars between the tony 25 144 00:02:27,030 --> 00:02:27,040 tony 25 145 00:02:27,040 --> 00:02:28,949 tony 25 concentration of regolith and the 50 146 00:02:28,949 --> 00:02:28,959 concentration of regolith and the 50 147 00:02:28,959 --> 00:02:31,990 concentration of regolith and the 50 concentration 148 00:02:31,990 --> 00:02:32,000 149 00:02:32,000 --> 00:02:35,509 for cotyledon emergence in five of the 150 00:02:35,509 --> 00:02:35,519 for cotyledon emergence in five of the 151 00:02:35,519 --> 00:02:36,309 for cotyledon emergence in five of the six 152 00:02:36,309 --> 00:02:36,319 six 153 00:02:36,319 --> 00:02:37,509 six cultivars with an exception of 154 00:02:37,509 --> 00:02:37,519 cultivars with an exception of 155 00:02:37,519 --> 00:02:39,430 cultivars with an exception of outrageous red romaine there was a large 156 00:02:39,430 --> 00:02:39,440 outrageous red romaine there was a large 157 00:02:39,440 --> 00:02:40,390 outrageous red romaine there was a large difference between the 158 00:02:40,390 --> 00:02:40,400 difference between the 159 00:02:40,400 --> 00:02:43,030 difference between the 10 and the 25 percent concentration of 160 00:02:43,030 --> 00:02:43,040 10 and the 25 percent concentration of 161 00:02:43,040 --> 00:02:45,030 10 and the 25 percent concentration of regolith 162 00:02:45,030 --> 00:02:45,040 regolith 163 00:02:45,040 --> 00:02:46,550 regolith and again for the first true leaves 164 00:02:46,550 --> 00:02:46,560 and again for the first true leaves 165 00:02:46,560 --> 00:02:48,630 and again for the first true leaves budding for five of the six cultivars 166 00:02:48,630 --> 00:02:48,640 budding for five of the six cultivars 167 00:02:48,640 --> 00:02:49,990 budding for five of the six cultivars the exception of the outrageous red 168 00:02:49,990 --> 00:02:50,000 the exception of the outrageous red 169 00:02:50,000 --> 00:02:51,509 the exception of the outrageous red romaine there's a large difference 170 00:02:51,509 --> 00:02:51,519 romaine there's a large difference 171 00:02:51,519 --> 00:02:52,229 romaine there's a large difference between 172 00:02:52,229 --> 00:02:52,239 between 173 00:02:52,239 --> 00:02:55,509 between the 25 and the 10 concentrations of the 174 00:02:55,509 --> 00:02:55,519 the 25 and the 10 concentrations of the 175 00:02:55,519 --> 00:02:58,149 the 25 and the 10 concentrations of the regolith 176 00:02:58,149 --> 00:02:58,159 177 00:02:58,159 --> 00:02:59,430 at harvest there was not enough 178 00:02:59,430 --> 00:02:59,440 at harvest there was not enough 179 00:02:59,440 --> 00:03:01,110 at harvest there was not enough vegetative tissue in order to collect 180 00:03:01,110 --> 00:03:01,120 vegetative tissue in order to collect 181 00:03:01,120 --> 00:03:01,830 vegetative tissue in order to collect data for 182 00:03:01,830 --> 00:03:01,840 data for 183 00:03:01,840 --> 00:03:05,110 data for the 50 concentration of regolith however 184 00:03:05,110 --> 00:03:05,120 the 50 concentration of regolith however 185 00:03:05,120 --> 00:03:07,830 the 50 concentration of regolith however for the 20 fresh weight for the 25 186 00:03:07,830 --> 00:03:07,840 for the 20 fresh weight for the 25 187 00:03:07,840 --> 00:03:09,589 for the 20 fresh weight for the 25 concentration of regolith relative 188 00:03:09,589 --> 00:03:09,599 concentration of regolith relative 189 00:03:09,599 --> 00:03:11,910 concentration of regolith relative to the control group each of the six 190 00:03:11,910 --> 00:03:11,920 to the control group each of the six 191 00:03:11,920 --> 00:03:12,710 to the control group each of the six cultivars 192 00:03:12,710 --> 00:03:12,720 cultivars 193 00:03:12,720 --> 00:03:14,149 cultivars was statistically significant in 194 00:03:14,149 --> 00:03:14,159 was statistically significant in 195 00:03:14,159 --> 00:03:17,030 was statistically significant in freshwater 196 00:03:17,030 --> 00:03:17,040 197 00:03:17,040 --> 00:03:19,910 for dry weight five of the six cultivars 198 00:03:19,910 --> 00:03:19,920 for dry weight five of the six cultivars 199 00:03:19,920 --> 00:03:21,589 for dry weight five of the six cultivars were statistically significant between 200 00:03:21,589 --> 00:03:21,599 were statistically significant between 201 00:03:21,599 --> 00:03:23,030 were statistically significant between the twenty-five percent concentration of 202 00:03:23,030 --> 00:03:23,040 the twenty-five percent concentration of 203 00:03:23,040 --> 00:03:24,149 the twenty-five percent concentration of burglars and the test 204 00:03:24,149 --> 00:03:24,159 burglars and the test 205 00:03:24,159 --> 00:03:27,509 burglars and the test in the control group 206 00:03:27,509 --> 00:03:27,519 207 00:03:27,519 --> 00:03:29,589 for the height for the harvest data it 208 00:03:29,589 --> 00:03:29,599 for the height for the harvest data it 209 00:03:29,599 --> 00:03:31,509 for the height for the harvest data it was very statistically significant in 210 00:03:31,509 --> 00:03:31,519 was very statistically significant in 211 00:03:31,519 --> 00:03:33,589 was very statistically significant in terms of the 25 percent 212 00:03:33,589 --> 00:03:33,599 terms of the 25 percent 213 00:03:33,599 --> 00:03:35,190 terms of the 25 percent concentration of regolith in which all 214 00:03:35,190 --> 00:03:35,200 concentration of regolith in which all 215 00:03:35,200 --> 00:03:36,630 concentration of regolith in which all six cultivars were statistically 216 00:03:36,630 --> 00:03:36,640 six cultivars were statistically 217 00:03:36,640 --> 00:03:37,509 six cultivars were statistically significant 218 00:03:37,509 --> 00:03:37,519 significant 219 00:03:37,519 --> 00:03:40,470 significant and in 10 concentration of regolith five 220 00:03:40,470 --> 00:03:40,480 and in 10 concentration of regolith five 221 00:03:40,480 --> 00:03:42,229 and in 10 concentration of regolith five of the six cultivars were statistically 222 00:03:42,229 --> 00:03:42,239 of the six cultivars were statistically 223 00:03:42,239 --> 00:03:43,030 of the six cultivars were statistically significant 224 00:03:43,030 --> 00:03:43,040 significant 225 00:03:43,040 --> 00:03:44,710 significant and as you can see pictured here the 226 00:03:44,710 --> 00:03:44,720 and as you can see pictured here the 227 00:03:44,720 --> 00:03:47,270 and as you can see pictured here the extra prok choi at zero percent 228 00:03:47,270 --> 00:03:47,280 extra prok choi at zero percent 229 00:03:47,280 --> 00:03:49,110 extra prok choi at zero percent um or the control group and the 10 230 00:03:49,110 --> 00:03:49,120 um or the control group and the 10 231 00:03:49,120 --> 00:03:53,830 um or the control group and the 10 concentration there is a wide difference 232 00:03:53,830 --> 00:03:53,840 233 00:03:53,840 --> 00:03:56,789 diameter was also greatly affected in 234 00:03:56,789 --> 00:03:56,799 diameter was also greatly affected in 235 00:03:56,799 --> 00:03:57,110 diameter was also greatly affected in for 236 00:03:57,110 --> 00:03:57,120 for 237 00:03:57,120 --> 00:04:00,229 for all six cultivars the 25 percent 238 00:04:00,229 --> 00:04:00,239 all six cultivars the 25 percent 239 00:04:00,239 --> 00:04:01,429 all six cultivars the 25 percent concentration of regolith was 240 00:04:01,429 --> 00:04:01,439 concentration of regolith was 241 00:04:01,439 --> 00:04:02,869 concentration of regolith was statistically significant from the 242 00:04:02,869 --> 00:04:02,879 statistically significant from the 243 00:04:02,879 --> 00:04:04,869 statistically significant from the control group 244 00:04:04,869 --> 00:04:04,879 control group 245 00:04:04,879 --> 00:04:07,190 control group what does this mean unamended mars 246 00:04:07,190 --> 00:04:07,200 what does this mean unamended mars 247 00:04:07,200 --> 00:04:08,309 what does this mean unamended mars global stimulant 1 248 00:04:08,309 --> 00:04:08,319 global stimulant 1 249 00:04:08,319 --> 00:04:10,869 global stimulant 1 severely inhibits vegetative crop growth 250 00:04:10,869 --> 00:04:10,879 severely inhibits vegetative crop growth 251 00:04:10,879 --> 00:04:12,550 severely inhibits vegetative crop growth in multiple cultivars 252 00:04:12,550 --> 00:04:12,560 in multiple cultivars 253 00:04:12,560 --> 00:04:15,030 in multiple cultivars and not just at 100 concentration this 254 00:04:15,030 --> 00:04:15,040 and not just at 100 concentration this 255 00:04:15,040 --> 00:04:17,990 and not just at 100 concentration this occurs at low concentrations as well 256 00:04:17,990 --> 00:04:18,000 occurs at low concentrations as well 257 00:04:18,000 --> 00:04:21,349 occurs at low concentrations as well and what this means is that this this 258 00:04:21,349 --> 00:04:21,359 and what this means is that this this 259 00:04:21,359 --> 00:04:23,270 and what this means is that this this regolith will need to be altered in some 260 00:04:23,270 --> 00:04:23,280 regolith will need to be altered in some 261 00:04:23,280 --> 00:04:25,830 regolith will need to be altered in some way in order to become a viable 262 00:04:25,830 --> 00:04:25,840 way in order to become a viable 263 00:04:25,840 --> 00:04:29,189 way in order to become a viable resource for crop growth on mars 264 00:04:29,189 --> 00:04:29,199 resource for crop growth on mars 265 00:04:29,199 --> 00:04:31,990 resource for crop growth on mars of course this was not done as one 266 00:04:31,990 --> 00:04:32,000 of course this was not done as one 267 00:04:32,000 --> 00:04:32,629 of course this was not done as one person 268 00:04:32,629 --> 00:04:32,639 person 269 00:04:32,639 --> 00:04:34,710 person science takes a lot of people and a lot 270 00:04:34,710 --> 00:04:34,720 science takes a lot of people and a lot 271 00:04:34,720 --> 00:04:36,390 science takes a lot of people and a lot of wonderful people contributed to this 272 00:04:36,390 --> 00:04:36,400 of wonderful people contributed to this 273 00:04:36,400 --> 00:04:36,950 of wonderful people contributed to this product 274 00:04:36,950 --> 00:04:36,960 product 275 00:04:36,960 --> 00:04:39,350 product project thank you so much for listening 276 00:04:39,350 --> 00:04:39,360 project thank you so much for listening 277 00:04:39,360 --> 00:04:43,840 project thank you so much for listening i hope you enjoy the project